Affiblot screening device for antibody selection

Technology description


The name of the technology: Affiblot screening device for antibody selection
Challenge: The increasing use of antibodies in various research fields (e.g. biotechnology) is closely related to their ever-expanding market portfolio. Thus, the selection of a suitable commercial antibody for a given application, which has appropriate affinity, high specificity, reproducibility, and low cross-reactivity, becomes crucial for this research area. A certain part of antibodies on the market does not fulfill the mentioned criteria therefore it is recommended to test the antibody before starting the experiments with them.
Description: - Compact device based on standard procedure dot blot technology
- Innovative lid with reagent reservoirs on the upper side and a pattern of drainage microchannels on the bottom side ensure all steps are inside the device
- Comparison of antibodies from multiple perspectives without cutting the membrane (against dot blot)
- Parallel evaluation of up to 5 antibodies
- Affinity/avidity comparison
- Determination of cross-reactivity with other biomolecules and whole cells (e.g. bacterial)
- Batch-to-batch comparison

Advantages of the technology:
- Only smartphone, vacuum pump, and software for densitometric evaluation are needed
- Speed of assay and reproducibility
- Simplicity in interpretation
- Antigen applied to the membrane can be both soluble (protein/peptide) and corpuscular (e.g. bacterial cell)
- Easy application, disposal of reagents, and blotting membrane
- Easy, reproducible, and precise SLA 3D printing of device
- Patented solution - reduction of time-consuming steps used in standard methods such as ELISA or Western blot

Commercial opportunity: The end users are all research teams and laboratories that work with antibodies or other affinity reagents.


IP protection status: Czech patent granted, doc. No. 308111
European patent granted, doc. No. 3669983
Development status:

Phase 2

Corresponds with TRL 3 and TRL 4

Feasibility study. There is a realistic design of the technology and the initial tests in the laboratory are leading to the specification of the technology requirements and its capabilities.

  • TRL 3 represents a complete idea of a technology with the design of concrete steps, tests and experiments that will need to be carried out to verify that the idea is realistic
  • TRL 4 is a verification of functionality in the laboratory. The result of TRL 4 is the so-called Proof-of-Concept. Technology is therefore designed and tested for its functionality under limited conditions
Partnering strategy: Co-development Collaboration licensing
More information: More information about the technology is provided in the publication: Affiblot: a dot blot-based screening device for selection of reliable antibodies (Anal. Methods, 2021, 13, 3874-3884). Use the QR code to download.
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Categories: Research Tools Diagnostics Life Science and Health Chemistry
Owner of a technology: Univerzita Pardubice, Ústav analytické chemie AV ČR Brno
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